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KMID : 0525720170220020103
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Journal of Chitin and Chitosan
2017 Volume.22 No. 2 p.103 ~ p.109
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Chitosan Oligosaccharides Inhibits LPS-Induced Inflammation Responses through the Phosphorylation of MAPK Singnal Pathways in Osteoblast Cell
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Song Kook
Kim Young-Ho
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Abstract
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The scaffold-based tissue engineering approach had become an important method for bone repair and regeneration. The basic requirements of scaffolds were non-toxic, non-inflammatory, biodegradable, absorbable, appropriate porous, and no side effects. In recent, chitosan-based scaffolds had been applied as a hydrogel. In the present study, we were to investigate the effect of chitosan oligosaccharides (COS) on lipopolysaccharide (LPS)-induced inflammation reaction and its molecular mechanisms in osteoblast. Less than 1,000 molecular weight and 90 to 95 percent deacetylated COS were used. Treatment of COS significantly inhibited LPS-production of nitric oxide (NO), Tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6) in a dose dependent manner with decreases in mRNA expression of interleukin-1β (IL-1β), TNF-α and IL-6. Furthermore, COS inhibited LPSinduced phosphorylation of inhibitory kappa B-α (IκB-α) and p-JNK, p38 mitogen-activated protein kinases (MAPKs) and also inhibited expression of toll-like receptor-4 (TLR-4). These results indicated that COS inhibited LPS-induced the inflammatory reaction of iNOS, TNF-α and IL-6 thorough down regulation of the TLR-4, nuclear factor-κB (NF-κB) and MAPKs pathway. It suggested that COS might be applied for developing an anti-inflammatory scaffold
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KEYWORD
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Chitosan oligosaccharide, Tumor necrosis factor-α, Interleukin-6, Nitric oxide, Nuclear factor-κB
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